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With the introduction of cutting-grown Petunia x hybrida plants on the European market, a new potyvirus which showed no serological reaction with antisera against any other potyviruses infecting petunias was discovered. A number of 'old standbys' an.Ĭharacterization of petunia flower mottle virus (PetFMV), a new potyvirus infecting Petunia x hybrida.įeldhoff, A Wetzel, T Peters, D Kellner, R Krczal, G A single cutting event on a TMV-infected plant was sufficient for transmission to many plants subsequently cut with the same clippers.
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Tobacco mosaic virus (TMV) and four other tobamoviruses infected multiple petunia cultivars without producing obvious viral symptoms.
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Surprising results from a search for effective disinfectants for Tobacco mosaic virus-contaminated tools Transformation efficiencies of around 10% are achievable with shoots being recovered about 8 wk after transgene insertion and rooted plants transferred to the greenhouse about twelve weeks after inoculation. Selection for transgenic plants is achieved using the bar gene conferring resistance to glufosinate or nptII gene for resistance to kanamycin. The system utilizes Agrobacterium tumefaciens for transgene integration with plant recovery via shoot organogenesis from leaf explant material. In this chapter, we describe a simple and reproducible genetic transformation protocol for generating transgenic petunia plants harboring a gene of interest and selectable marker.
Rdr2 meteorite update#
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The bursts to the body, leaving its way over the skin, and causing Chill status, reducing the speed of attack and movement by 60.A giant pillar then raises from the target area and places foes in the air and explodes. Executive: Take meteor fragments from the sky so they can cause damage to enemies.Some of her spells include: “The one who’s doing something like this one” In addition, players have many exciting changes coming into the game that will make it unique in the west, as well as Asian counterparts.įirst off, please look at the Sorceress, a formidable caster that works well on ice, fire, and lightning. When the games start in January, the Sorceress class will join the lineup, which includes 15 unique classes. It would be best for fans that want even more reasons for playing Lost Ark.